Pakt immunofluorescence
WebOct 9, 2014 · To investigate if Mapk/Erk and PI3K/Akt signaling are also restricted to MG-derived progenitors, we investigated pErk and pAkt expression in the uninjured and injured retina using immunofluorescence. Interestingly, pErk and pAkt increased in MG and photoreceptor outer segments, respectively, throughout the retina within 1 hpi (Figure S3 …
Pakt immunofluorescence
Did you know?
WebApr 9, 2011 · Immunofluorescence Confocal Analysis CRC cells grown on glass coverslips were exposed to the indicated concentrations of gefitinib, AG1024, or both. After incubation for 3 h, covered cells were rinsed with PBS, fixed in 4% paraformaldehyde at room temperature for 20 min, and then permeabilized with ice-cold 2.5% Triton for 3–5 min. WebT308 pAKT immunofluorescence was present in neuronal cytoplasm and nuclei in the HMEG/FCD specimens with identified PIK3CA, AKT3 and PTEN mutations . Neuronal T308 pAKT immunofluorescence was also present in many HMEG/FCD specimens without detected mutations, consistent with western blot results (Fig. 3 and Supplementary Fig. 4).
WebQuantitative proteomics analysis confirmed that the protein cargo of EVs are modified upon activation of PI3K/Akt signaling pathway. Bioinformatics analysis highlighted the … WebPlatelet detection allows laboratories to determine compatibility between patient and donor prior to platelet transfusion. Our complete portfolio includes a variety of products for …
WebJun 1, 2024 · 2.2. Immunofluorescence (IF) IF was performed on paraffin embedded placental sections as previously performed in our laboratory [20].Briefly, serial sections were incubated overnight with rabbit polyclonal antibodies against phospho (p) AKT, pERK, pmTOR, pp70, p4EBP1(all from cell Signaling, Danvers, MA) or cytokeratin 7 (for … WebConfocal immunofluorescent analysis of C2C12 cells, LY294002-treated (left) or insulin-treated (right), using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb (green). Actin …
WebJul 11, 2024 · We describe a tissue-based cyclic immunofluorescence (t-CyCIF) method for highly multiplexed immuno-fluorescence imaging of formalin-fixed, paraffin-embedded (FFPE) specimens mounted on glass slides, the most widely used specimens for histopathological diagnosis of cancer and other diseases. t-CyCIF generates up to 60 …
WebImmunogen Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser473 of human Akt. Validations … ews1 building heightWebApr 5, 2024 · Immunofluorescence microscopy. EDL and SOL muscles stored in OCT compound were cut into 10 μm thick sections at −20°C on a cryostat (Leica Biosystems), collected on Superfrost Plus Gold slides, and stored at −80°C. ... The expression and phosphorylation status of pAkt Ser473 were not impacted by MK, however, total Akt was … bruised back pain from fallWebKey features and details Rabbit polyclonal to pan-AKT Suitable for: IHC-P, WB, ICC/IF Reacts with: Mouse, Rat, Human Isotype: IgG Get better batch-to-batch reproducibility with a recombinant antibody Anti-AKT1 + AKT2 antibody [EPR18405] (ab188099) Research with confidence – consistent and reproducible results with every batch bruised back treatmentWebThe DNA dye DAPI in combination with immunofluorescence and confocal microscopy were used to identify the nucleated cells (blue fluorescence) that contained pAkt (red fluorescence) in... bruised armpits after waxingWebOct 24, 2016 · Immunofluorescence and chromatin immunoprecipitation (ChIP)–quantitative PCR (qPCR) showed that MOZ and H3K14cr colocalize in a DPF-dependent manner. Our studies call attention to a new... ews1 certificate costWebMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser473 of mouse Akt. Background Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). bruised back of heel boneWebIn parallel with RAD001-induced inhibition of cell viability, a dose- and schedule- dependent down-regulation of pAKT and p-eIF4E expressions was demonstrated. In contrast, a … bruised back ribs treatment