2024 Electroporation of phaeodactylum tricornutum

Electroporation of phaeodactylum tricornutum

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August undefined, 2024

WebThe diatom P. tricornutum, shown in figure 1, was purchased from the CCAP algae collection and grown in purified domestic seawater mixed with Guillard’s f/2 medium recipe (Guillard, 1975), rather standard in cultivation of marine diatoms, ingredients are shown in table 1. Figure 1. Phaeodactylum tricornutum Bohlin (1897). CCAP 1055/15. WebHighly efficient transformation of the diatom Phaeodactylum tricornutum by multi-pulse electroporation Epub 2013 Apr 7. Authors Mado Miyahara 1 , Masaki Aoi , Natsuko Inoue-Kashino , Yasuhiro Kashino , Kentaro Ifuku Affiliation 1 Graduate School of Biostudies, Kyoto University, Kyoto, Japan. PMID: 23563551 DOI: 10.1271/bbb.120936 …

Highly Efficient Transformation of the Diatom Phaeodactylum …

WebHerein reported are new tricyclic cytidine compounds, such as 8-diethylamino-tC (8-DEA-tC), that respond to DNA and/or RNA duplex formation with up to a 20-fold increase in fluore WebApr 1, 2024 · In this study, the correlation between the specific growth rate of Phaeodactylum tricornutum and fucoxanthin (fucoxanthin) biosynthesis was investigated both in batch and in semi-continuous cultures. Fucoxanthin content from P. tricornutum biomass showed a positive correlation with specific growth rate during the … black bear profile

Marine Drugs Free Full-Text Promoter Trapping in Microalgae …

WebJul 15, 2024 · Phaeodactylum tricornutum is a marine pennate species that has become an experimental model used to understand diatom biology. The nuclear genome is chimeric, containing genes derived from ancestral endosymbioses with red and green algae, and enriched by genes acquired laterally from bacteria. WebDec 22, 2015 · In addition, methods of genetic transformation via biolistic bombardment and/or electroporation have been developed for several diatom species including Pennales P. tricornutum 10,11,12,13,14,15 ... Web宋燕子贾彬林柏成胡章立黄瑛（深圳市海洋生物资源与生态环境重点实验室深圳市海洋藻类开发与应用工程实验室深圳大学生命科学学院，深圳 518060） black bear prints in mud

Highly efficient transformation of the diatom Phaeodactylum tricornutum ...

Phaeodactylum tricornutum - an overview ScienceDirect Topics

WebKey words: diatom; electroporation; multi-pulse; Phaeodactylum tricornutum Diatoms are important primary producers in the ecosystem that have crucial roles in the global carbon … WebAug 27, 2024 · Phaeodactylum tricornutum (P. tricornutum) is a marine diatom 5–27 μm in length and 3–4 μm in width belonging to the Phaeodactylaceae family. P. tricornutum can be found in different morphotypes (i.e., fusiform, triradiate and oval) that may have different behaviors under different downstream processing conditions. galactosemia associationsWebThe diatom P. tricornutum, shown in figure 1, was purchased from the CCAP algae collection and grown in purified domestic seawater mixed with Guillard’s f/2 medium … galactose functional groups

"WebJun 1, 2012 · In a study using microalgae Phaeodactylum tricornutum, the pHY18 vector was ligated with gene of interest DGAT2 and then transformed by electroporation … " - Electroporation of phaeodactylum tricornutum

Electroporation of phaeodactylum tricornutum

Construction of Novel Chloroplast Expression Vector and

WebJul 26, 2024 · Fucoxanthin amount has been shown to double in engineered P. tricornutum overexpressing the gene encoding lycopene-β-cyclase (LCYB) under the control of the fcpB promoter, when fed with basal... WebMay 26, 2009 · Phaeodactylum tricornutum is the most promising diatom exploited to date, especially as a platform of pharmaceutical production. Herein, we expose diatoms’ main features that allowed using them ...

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WebAn efficient nuclear transformation method has been established for the pennate marine diatom Phaeodactylum tricornutum using an electroporation system that drives multisequence pulses to introduce foreign DNAs into the cells. By removing excess salts in the culture medium and optimizing pulse condi … WebApr 3, 2024 · The promoter/terminator of the nitrate reductase gene of the model diatom Phaeodactylum tricornutum was cloned and used to drive chloramphenicol acetyltransferase (CAT) reporter gene expression. The construct was transferred by electroporation into P. tricornutum grown in medium lacking silicon. CAT expression …

WebHolly L. Prentice's 9 research works with 530 citations and 118 reads, including: Mutations in the Schizosaccharomyces pombe heat shock factor that differentially affect responses to heat and ... WebApr 23, 2013 · A highly efficient nuclear transformation method was established for the pennate diatom Phaeodactylum tricornutum using an electroporation system that …

WebJan 1, 2024 · In this study, the marine diatom Phaeodactylum tricornutum was selected as the model organism since it presents many advantages, including culture easiness and … WebGenetic transformation system is very important for both basic biological research and commercial exploitation of diatoms. Here we describe a high-efficiency nuclear …

WebAug 30, 2024 · An efficient nuclear transformation method has been established for the pennate marine diatom Phaeodactylum tricornutum using an electroporation system …

WebCell Size-specific Transfection by Micropillar Array Electroporation Xuan Liu, Yingbo Zu, and 385535540195. ELEKTROPORATIONSPROTOKOLLE: MIKROORGANISMUS, SÄUGETIERSYSTEM und Nanogerät von Sh - EUR 297,94. ZU VERKAUFEN! Cell Size-specific Transfection by Micropillar Array Electroporation Xuan Liu, Yingbo Zu, and … blackbearproperties株式会社Web• Developed an electroporation protocol for gene transformation in diatoms. ... Quantification of chrysolaminarin from the model diatom Phaeodactylum tricornutum Algal research December 31, ... black bear propane black diamondWebPhaeodactylum tricornutum (UTCC646). We attempted a trophic conversion of this alga by transforming it with genes encoding glucose transporters. The transporter genes used in-cluded Glut1 from human erythrocytes (12); Hup1 from the microalga Chlorella kessleri (13); and Hxt1, Hxt2, and Hxt4 from Saccha-romyces cerevisiae (14). The coding regions black bear propane spanaway waWebA highly efficient nuclear transformation method was established for the pennate diatom Phaeodactylum tricornutum using an electroporation system that drives multi … black bear propane spanawayWebDec 4, 2012 · As concerns the classical microalgal transformation systems referred, Chlamydomonas and Phaeodactylum, there is a wide collection of strong constitutive homologous promoters, such as the rubisco small subunit RBCS2 promoter fused to the heat shock protein (HSP70A) promoter of C. reinhardtii or the fucoxanthin chlorophyll … galactose and glucose bondWebP. tricornutum strain CCAP1055/1 was used in this study. The LCYB sequence was amplified from cDNA synthesized from total RNA, and cloned into plasmid pPha-T1 [9]. P. tricornutum transformation was carried out using a simple electroporation protocol described by Zhang and colleagues [10]. For the analysis of growth conditions effects on ... galactosemia in chineseWebGenetic transformation system is very important for both basic biological research and commercial exploitation of diatoms. Here we describe a high-efficiency nuclear … galactose chinese