WebJun 28, 2016 · Cells and transfection . The human cervical cancer cell line SiHa was purchased from the American Type Culture Collection (Manassas, VA, USA) and cultured in Dulbecco's modified Eagle's medium (DMEM; Hyclone; GE Healthcare Life Sciences, Beijing, China) supplemented with 10% fetal bovine serum (Hyclone; GE Healthcare Life … WebMice were injected intraperitoneally with 2 mL of 3.5% sterile thioglycollate solution (BD, Sparks, MD, USA); 4 days later, mice were sacrificed by cervical dislocation, and cells were isolated by peritoneal lavage with cold DMEM (Hyclone, Logan, UT, USA).

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WebHEK 293T were cultured in Dulbecco’s modified Eagle’s medium (DMEM; HyClone) supplemented with 10% (v/v) FBS and 1% (v/v) P/S. All cells were cultured at 37 °C in a humidified ... (Invitrogen, USA), according to the manufacturer’s protocol. Reverse transcription was performed using the PrimeScript First-Strand cDNA Synthesis Kit … WebVSMCs were cultured in basal DMEM/F12 medium (HyClone, Logan, Utah, USA), HUVECs were grown in RPMI 1640 medium (HyClone, Logan, Utah, USA), and 293 T cells were maintained in DMEM/high-glucose medium (HyClone, Logan, Utah, USA). The complete culture medium was supplemented with 10% fetal bovine serum (HyClone, Logan, Utah, … eline the razor won\\u0027t die

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